Results of twenty-year studies on immunity at preclinical asymptomatic phase of developing type 1 diabetes in children on the program IPDM: 1. Leukocyte composition and immune phenotype of blood lymphocytes
Vol. 22 No. 3 (2017), ORIGINAL PAPERS
Vol. 22 No. 3 (2017)

Results of twenty-year studies on immunity at preclinical asymptomatic phase of developing type 1 diabetes in children on the program IPDM: 1. Leukocyte composition and immune phenotype of blood lymphocytes

ORIGINAL PAPERS
Published September 28, 2017
K.P. Zak
SI «V.P. Komisarenko Institute of Endocrinology and Metabolism, Natl. Acad. Sci. of Ukraine»
V.V. Popov
SI «V.P. Komisarenko Institute of Endocrinology and Metabolism, Natl. Acad. Sci. of Ukraine»
M.A. Gruzov
SI «V.P. Komisarenko Institute of Endocrinology and Metabolism, Natl. Acad. Sci. of Ukraine»
B.M. Khomenko
SI «V.P. Komisarenko Institute of Endocrinology and Metabolism, Natl. Acad. Sci. of Ukraine»
V.V. Afanasyeva
SI «V.P. Komisarenko Institute of Endocrinology and Metabolism, Natl. Acad. Sci. of Ukraine»
T.N. Malynovskaya
SI «V.P. Komisarenko Institute of Endocrinology and Metabolism, Natl. Acad. Sci. of Ukraine»
E.N. Tronko
SI «V.P. Komisarenko Institute of Endocrinology and Metabolism, Natl. Acad. Sci. of Ukraine»
Ya.A. Saenko
SI «V.P. Komisarenko Institute of Endocrinology and Metabolism, Natl. Acad. Sci. of Ukraine»
T.A. Semionova
SI «V.P. Komisarenko Institute of Endocrinology and Metabolism, Natl. Acad. Sci. of Ukraine»
A.B. Kulskovskaya
SI «V.P. Komisarenko Institute of Endocrinology and Metabolism, Natl. Acad. Sci. of Ukraine»
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Keywords

type 1 diabetes mellitus, leukocyte composition, immunophenotype of blood lymphocytes

How to Cite

Zak, K., Popov, V., Gruzov, M., Khomenko, B., Afanasyeva, V., Malynovskaya, T., Tronko, E., Saenko, Y., Semionova, T., & Kulskovskaya, A. (2017). Results of twenty-year studies on immunity at preclinical asymptomatic phase of developing type 1 diabetes in children on the program IPDM: 1. Leukocyte composition and immune phenotype of blood lymphocytes. Endokrynologia, 22(3), 201-210. Retrieved from https://endokrynologia.com.ua/index.php/journal/article/view/77
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Abstract

Aim. To study the leukocyte composition and immunophenotype of blood lymphocytes in normoglycemic children with burdened heredity, who are in asymptomatic phase of developing type 1 diabetes mellitus (type 1 DM), which was established on the basis of determining the autoantibody titre to pancreatic islets of Langerhans (OAA). Methods. There were examined 457 normoglycemic children with burdened heredity to type 2 DM registered in the register of national program «Immunity in Pre-clinical Stage of Diabetes Mellitus Development » (IPDM), which first line relatives had type 1 DM, divided into four sub-groups (1 — healthy without genetic predisposition to type 1 DM; 2 — OAA-negative; 3 — OAA-positive; 4 — patients with type 1 DM). Determination of leukocyte formula was traditionally performed and by hematological analyzer, immunophenotype of lymphocytes (CD3+T, CD4+T, CD8+T, CD20+, CD56+ cells) — by flow cytometry (FACS analysis) and the titer of OAA (IAA, GADA and IA-2A) — by radioimmunological method. Results. It was found that a pronounced change in a natural (neutrophilepenia, decrease in a number and activity of NK-cells (CD56+ lymphocytes and BGL)) as well as adaptive immunity (reduction in relative and absolute number of CD3+T, CD4+T, CD8+T and, especially, immunoregulatory subpopulation of CD4+T cells), compared with OAA-negative group of children with burdened
heredity (p<0.05), and a group of healthy normoglycemic genetically uncomplicated children (p<0.001). Conclusion. The marked changes in natural and adaptive immunity, corresponding to the new concept of the pathogenesis and management of type 1 DM in asymptomatic pre-clinical phase of type 1 DM development in children are occured.
This concept is that the therapy of type 1 DM should not begin at the stage of a sharp decrease in the beta cells mass and the insulin secretion, i.e. after an appearance of pathological clinical signs, but in the asymptomatic phase, when the autoimmune process in the islets has just begun.

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References

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