Keywords
potassium ions, lithium ions, adrenocorticocytes, glycogen synthase kinase-3β.
How to Cite
Kovzun, O., Levchuk, N., Pushkarev, V., Pushkarev, V., & Tronko, M. (2013). Effect of potassium ions on the level of expression of glycogen synthase kinase in guinea pigs adrenocorticocytes. Endokrynologia, 18(4), 11-15. Retrieved from https://endokrynologia.com.ua/index.php/journal/article/view/355
Abstract
The influence of potassium ions – steroidogenesis aсtivator in adrenocorticocytes, and lithium ions – glycogen synthase kinase-3beta (GSK-3β) inhibitor, on GSK-3β expression was studied. GSK-3β is an important regulator of apoptosis, proliferative processes, differentiation, glucose transport and other biochemical processes. For the first time it has been shown that K+ has a profound effect on the level of protein kinase synthesis. The amount of GSK-3β decreases markedly at high concentrations of potassium, which stimulate steroidogenesis in adrenocorticocytes (5.5 and 8.5 mmol/l), and it significantly
increases in case of potassium deficiency in the medium. Lithium ions dosage-dependently enhance kinase expression. The authors discuss the mechanisms of potassium action on the expression of GSK-3β in adrenocorticocytes and possible involvement of this kinase in steroidogenesis.
References
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3. Yeste-Velasco M., Folch J., Trullas R. et al. Glycogen synthase kinase-3 is involved in the regulation of the cell cycle in cerebellar granule cells // Neuropharmacology. 2007, 53, N 2, 295-307.
4. Iqbal K., Grundke-Iqbal I. Alzheimer neurofibrillary degeneration: significance, etiopathogenesis, therapeutics and prevention // J. Cell Mol. Med. 2008, 12, N 1, 38-55.
5. Holmes T., O’Brien T.A., Knight R. et al. The role of glycogen synthase kinase-3beta in normal haematopoiesis, angiogenesis and leukaemia // Curr. Med. Chem. 2008, 15, N 15, 1493-1499.
6. Garcea G., Manson M.M., Neal C.P. et al. Glycogen synthase kinase-3 beta; a new target in pancreatic cancer? // Curr. Cancer Drug Targets. 2007, 7, N 3, 209-215.
7. Takahashi-Yanaga F., Sasaguri T. GSK-3beta regulates cyclin D1 expression: a new target for chemotherapy // Cell Signal. 2008, 20, N 4, 581-589.
8. Malhi G. S., Tanious M., Das P., Berk M. The science and practice of lithium therapy // Australian N. Zealand J. Psychiatry. 2012, 46, 192-211.
9. Bschor T., Ritter D., Winkelmann P. et al. Lithium monotherapy increases ACTH and cortisol response in the Dex/CRH test in unipolar depressed subjects. A study with 30 treatment-naive patients // PLoS One. 2011, 6, N 11, e27613, 1-8.
10. Тронько М.Д., Ковзун О.І., Пушкарьов В.М. Механізми ре- гуляції стероїдогенезу в корі надниркових залоз. К.: ЦНЛ, 2006. 304 с. (Tronko M.D., Kovzun O.I., Pushkarev V.M. The mechanisms of regulation of steroidogenesis in the adrenal cortex. Kiev: CNL, 2006. 304 p.).
11. Gross E.R., Hsu A.K., Gross G.J. GSK3beta inhibition and K(ATP) channel opening mediate acute opioid-induced cardioprotection at reperfusion // Basic Res. Cardiol. 2007, 102, N 4, 341-349.
12. Yeste-Velasco M., Folch J., Casadesus G. et al. Neuroprotection by c-Jun NH2-terminal kinase inhibitor SP600125 against
potassium deprivation-induced apoptosis involves the Akt pathway and inhibition of cell cycle reentry. Neuroscience // 2009, 159, N3, 1135-1147.
13. Wada A. Lithium and neuropsychiatric therapeutics: neuroplasticity via glycogen synthase kinase-3beta, betacatenin, and neurotrophin cascades // J. Pharmacol. Sci. 2009, 110, N 1, 14-28.
14. Пушкарьов В.М. Біохімічні механізми регуляції стероїдогенезу в корі надниркових залоз іонами калію. Дис. докт. біол. наук. К., 2005. 329 с. (Pushkarev V.M. Biochemical mechanism of steroidogenesis regulation in adrenal cortex by potassium ions. Dissertation for the obtaining of a scientific degree of Doctor of Biological Sciences. Kyiv, 2005. 329 p.).
15. Ковзун Е.И., Лукашеня О.С., Пушкарев В.М. и др. Влияние ионов калия и лития на экспрессию NO-синтаз в коре надпочечников человека // Бюлл. эксп. биол. мед. 2013, № 9, 307-309. (Kovzun Ye.I., Lukashenya O.S., Pushkarev V.M. et al. Effect of potassium and lithium ions on NO-syntases expression in the human adrenal cortex // Bulletin of Experim. Biol. and Med. 2013, N 9, 307-309).
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