Activation of extracellular signal-regulated kinase‑1/2 in blood mononuclear cells of patients with diabetes and autoimmune thyroiditis
Vol. 25 No. 1 (2020), ORIGINAL PAPERS
Vol. 25 No. 1 (2020)

Activation of extracellular signal-regulated kinase‑1/2 in blood mononuclear cells of patients with diabetes and autoimmune thyroiditis

ORIGINAL PAPERS
https://doi.org/10.31793/1680-1466.2020.25-1.49
Published March 20, 2020
V.V. Pushkarev
State Institution «V.P. Komisarenko Institute of Endocrinology and Metabolism of the NAMS of Ukraine»
https://orcid.org/0000-0001-5940-5510
L.K. Sokolova
State Institution «V.P. Komisarenko Institute of Endocrinology and Metabolism of the NAMS of Ukraine»
https://orcid.org/0000-0003-0011-0106
O.I. Kovzun
State Institution «V.P. Komisarenko Institute of Endocrinology and Metabolism of the NAMS of Ukraine»
https://orcid.org/0000-0002-6906-6636
S.A. Cherviakova
ДУ «Інститут ендокринології та обміну речовин ім. В.П. Комісаренка НАМН України»
T.S. Vatseba
ДУ «Інститут ендокринології та обміну речовин ім. В.П. Комісаренка НАМН України»
V.M. Pushkarev
State Institution «V.P. Komisarenko Institute of Endocrinology and Metabolism of the NAMS of Ukraine»
https://orcid.org/0000-0003-0347-7771
M.D. Tronko
State Institution «V.P. Komisarenko Institute of Endocrinology and Metabolism of the NAMS of Ukraine»
https://orcid.org/0000-0001-7421-0981
pdf (Українська)

Keywords

peripheral blood mononuclear cells, extracellular signal-regulated kinase‑1/2, type 1 and type 2 diabetes, cancer, autoimmune thyroiditis.

How to Cite

Pushkarev, V., Sokolova, L., Kovzun, O., Cherviakova, S., Vatseba, T., Pushkarev, V., & Tronko, M. (2020). Activation of extracellular signal-regulated kinase‑1/2 in blood mononuclear cells of patients with diabetes and autoimmune thyroiditis. Endokrynologia, 25(1), 49-52. https://doi.org/10.31793/1680-1466.2020.25-1.49
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Abstract

The peripheral blood mononuclear cells (PBMC) mainly includes monocytes and lymphocytes involved in the development of diabetes mellitus and other autoimmune diseases. Ret/Ras/Raf/MEK/ERK is a signaling cascade that controls cellular processes such as proliferation, survival, angiogenesis, cell growth and motility. The aim of the work was to study the activation of the main effector protein kinase of this cascade — ЕRК1/2 in PBMC of patients with diabetes and autoimmune (Hashimoto’s) thyroiditis. Material and methods. The material of this work was the blood of healthy subjects, patients with type 2 diabetes, with type 2 diabetes and AIT, with type 2 diabetes and cancer (papillary thyroid carcinoma), with type 1 diabetes, with type 1 diabetes and AIT. Enzyme-linked immunosorbent assay kit 85-86012 («Invitrogen», USA) were used to determine the amount of phospho-ЕRК1/2 (p-Thr202/Thr204, Thr185/ Tyr187, respectively). Results. Patients were divided into 6 groups: 1 — control — healthy subjects, representative for age and BMI, 2 — patients with type 2 diabetes, 3 — patients with type 2 diabetes and AIT, 4 — patients with type 2 diabetes and thyroid cancer, 5 — patients with type 1 diabetes, 6 — patients with type 1 diabetes and AIT. It was shown that activation of ЕRК1/2 in PBMC of patients with type 2 diabetes and cancer was not observed, while in patients with type 1 diabetes or autoimmune thyroiditis it increased significantly. However, in patients with type 1 diabetes with autoimmune thyroiditis, the activation of ЕRК1/2 in PBMC decreased to a control level, which can be explained by the competition between the two autoimmune processes for common signaling pathways. Conclusion. In PBMC of patients with autoimmune diseases (type 1 diabetes or AIT), MAPK/ERK cascade is activated.

https://doi.org/10.31793/1680-1466.2020.25-1.49
pdf (Українська)

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