Combined effect of docetaxel and human-beta-defensis-2 on viability of follicular thyroid cancer cells
Vol. 20 No. 1 (2015), ORIGINAL PAPERS
Vol. 20 No. 1 (2015)

Combined effect of docetaxel and human-beta-defensis-2 on viability of follicular thyroid cancer cells

ORIGINAL PAPERS
Published March 15, 2015
O.L. Gerashchenko
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
O.V. Zhuravel
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
M.O. Soldatkina
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
V.M. Pushkarev
State Institution «V.P. Komisarenko Institute of Endocrinology and Metabolism. Natl. Acad. Med. Sci. of Ukraine»
P.V. Pogrebnoy
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
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Keywords

follicular thyroid cancer
docetaxel
cell viability
recombinant human beta-defensin-2

How to Cite

Gerashchenko, O., Zhuravel, O., Soldatkina, M., Pushkarev, V., & Pogrebnoy, P. (2015). Combined effect of docetaxel and human-beta-defensis-2 on viability of follicular thyroid cancer cells. Endokrynologia, 20(1), 391-395. Retrieved from https://endokrynologia.com.ua/index.php/journal/article/view/200
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Abstract

The effect of low docetaxel (Dtx) concentrations in combination with recombinant human beta-defensin-2 (rec-hBD-2) on viability of cultured follicular thyroid  carcinoma WRO cells has been studied. Treatment with 0.1 nM Dtx caused insignif cant decrease of WRO cell viability while 1 nM Dtx caused statistically signif cant  cytotoxic effect. Incubation of WRO cells with 0.1 or 1 nM Dtx resulted in signif cant up-regulation of key proteins involved in cell cycle control, in particular, cyclin D1,  p53 protein, and p21Waf1/Cip1. Treatment of WRO cells with 10 nM rec-hBD-2 resulted in stimulation of cell viability, while their incubation with 100 nM rec-hBD-2  resulted in signif cant decrease of cell viability. The most signif cant suppression of WRO cell viability was observed in the case of combined use of 1 nM rec-hBD-2 and 0.1 nM Dtx. So, we have  shown that in cultured follicular thyroid cancer cells mitogenic concentrations of rec-hBD-2 could potentiate growth-suppressive effects of the lowest Dtx concentrations.  

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Copyright (c) 2015 SI ≪V.P. Komisarenko Institute of Endocrinology and Metabolism, Natl. Acad. Med. Sci. of Ukraine≫

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