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Abstract
The flutamide usage is limited by its hepatotropic effects, especially due to its ability to inhibit specific synthetic and transport processes in the liver. Among variety liver functions, an important role is given to lipid metabolism and bile formation. Optimal physiologic ratio of the bile lipid components and lipid content in the blood are modified by various factors that may cause dysfunctions of bile formation and secretion. Aim. The aim of this study was to investigate flutamide effect on male rat bile lipid components (cholesterol, phospholipids, fatty acids and triglycerides) concentrations. Materials and methods. Research was carried out on fourteen Wistar male rats (0.18-0.23 kg) of local breeding. In experimental group flutamide was administered intragastrically daily at a dose of 25 mg/kg for 3 days. Control animals received flutamide vehicle. Six half-hour samples of rat
bile were collected in an acute experiments under thiopental anesthesia (60 mg/kg). The concentrations of phospholipids, cholesterol, free fatty acids, triglycerides, cholesterol esters and bile acids in rat bile were defined by TLC. There were calculated the bile acids / cholesterol ratio, the rate of cholesterol esterification, the phospholipids / total cholesterol ratio in bile. Statistical analysis of the results was done with Statistica 7.0 (Stat Soft, USA). Statistical significance of the data differences was calculated by Student’s t-test. Results. Flutamide reduced concentration of the bile phospholipids, while caused an increase in the triglyceride content. Under the influence of flutamide the ratio phospholipids /
total cholesterol decreased, but the bile acids / cholesterol and the cholesterol / cholesterol esters ratios remained unchanged. Conclusions. The shot-term of flutamide treatment of male rats causes a decrease in the bile concentration of phospholipids and phospholipid / total cholesterol ratio. It is reasonable to study effects of its chronic administration in both sexes and to estimate their sex-related differences.
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