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Abstract
Сhildren are often subjected to psychological or physical violence, experience strong emotional distress. Early life stress may lead to disorders of somato-sexual development, to delayed (in boys) or accelerated (in girls) puberty, delayed growth, immune system disorders, alteration of mental health. The sexual performance of adult men who experienced chronic stress during adolescence is not reflected in the scientific medical literature. Deferred effect of stress at adolescence on the androgen profile and sexual behavior of laboratory male animals has been studied very poorly. There are no data on long-term oxidative effects of stress experienced by human and animal adolescents in relation to the organs of the reproductive system. The aims of this work were to explore the sexual behavior, testosterone levels in blood and lipid peroxidation (LPO) in the testes of adult male rats in a distant period of time after chronic immobilization stress during puberty. White male rats were subjected to immobilization in plastic tubes for 1 hour a day during postnatal days 30-45. The non-stressed animals were used as controls. Some rats were decapitated at the age of 6 months, and the trunk blood samples were collected for hormone assay. Testosterone levels in blood serum were determined by immunoassay. The testicles were isolated to determine the content of the LPO products. At 7 months of age, the males were tested for the exhibition of male-type sexual behavior, and at 8 months for female-type sexual behavior after orchiectomy and priming with estradiol and progesterone. In stressed at puberty adult rats, testosterone levels did not differ from those of controls. Pubertal stress significantly reduced latency of the first and second mounts, that is increased the motivational component of male sexual behavior without increasing the number of mounts and intromissions. Stressed rats did not exhibit lordosis reaction in the presence of active male. As a result of stress in rats during puberty, the content of malonic dialdehyde and diene conjugates increased in the testicles of adult animals, which indicates the presence of oxidative stress.
References
Strashok LA, Rak LI, Danylenko HM, Yeshchenko AV, Kashina- Yarmak VL, Zavelya EM, et al. Impact of stress on adolescents during puberty (part 1). Child`s Health. 2023;18(5):376-83. doi: 10.22141/2224-0551.18.5.2023.1616. Ukrainian.
Danylenko GM, Strashok LA, Zavelja EM, Isakova MJu, Bjeljajeva OE. Medical and social aspects of the health status of the adolescents-internally displaced persons. Children and Adolescents Health Care. 2022;(2):8-12. doi: 10.53457/2022.2.1. Ukrainian.
Harris EP, Villalobos-Manriquez F, Melo TG, Clarke G, O’Leary OF. Stress during puberty exerts sex-specific effects on depressive-like behavior and monoamine neurotransmitters in adolescence and adulthood. Neurobiol Stress. 2022 Oct 3;21:100494. doi: 10.1016/j.ynstr.2022.100494.
Strashok LA, Rak LI, Danylenko HM, Yeshchenko AV, Kashina-Yarmak VL, Zavelya EM, et al. Impact of stress on adolescents during puberty (part 2). Child`s Health. 2023;18(6):465-73. doi: 10.22141/2224-0551.18.6.2023.1635. Ukrainian.
Reznikov AG. Stress-induced disorders of reproductive functions. Fiziol Zh. 2023;69:97-107. doi: 10.15407/fz69.06.097. Ukrainian.
Gleason JL, Shenassa ED, Thoma ME. Stressful life events, the incidence of infertility, and the moderating effect of maternal responsiveness: a longitudinal study. J Dev Orig Health Dis. 2021 Jun;12(3):465-73. doi: 10.1017/S2040174420000690.
Sophie Gibson ME, Fleming N, Zuijdwijk C, Dumont T. Where have the periods gone? The evaluation and management of functional hypothalamic amenorrhea. J Clin Res Pediatr Endocrinol. 2020 Feb 6;12(Suppl1):18-27. doi: 10.4274/jcrpe.galenos. 2019.2019.S0178.
Hager M, Dewailly D, Marculescu R, Ghobrial S, Parry JP, Ott J. Stress and polycystic ovarian morphology in functional hypothalamic amenorrhea: a retrospective cohort study. Reprod Biol Endocrinol. 2023 May 11;21(1):42. doi: 10.1186/s12958-023-01095-5.
Yano K, Matsuzaki T, Iwasa T, Mayila Y, Yanagihara R, Tungalagsuvd A, et al. The influence of psychological stress in early life on sexual maturation and sexual behavior in male and female rats. Reprod Med Biol. 2019 Dec 26;19(2):135-41. doi: 10.1002/rmb2.12313.
Hernández-Arteaga E, Hernández-González M, Bonilla-Jaime H, Guevara MA, Ågmo A. Pubertal stress decreases sexual motivation and supresses the relation between cerebral theta rhythms and testosterone levels in adult male rats. Brain Res. 2020 Oct 15;1745:146937. doi: 10.1016/j.brainres.2020.146937.
Almeida SA, Kempinas WG, Lamano Carvalho TL. Sexual behavior and fertility of male rats submitted to prolonged immobilization-induced stress. Braz J Med Biol Res. 2000 Sep;33(9):1105-9. doi: 10.1590/s0100-879x2000000900019.
Holson RR, Gough B, Sullivan P, Badger T, Sheehan DM. Prenatal dexamethasone or stress but not ACTH or corticosterone alter sexual behavior in male rats. Neurotoxicol Teratol. 1995 Jul-Aug;17(4):393-401. doi: 10.1016/0892-0362(94)00074-n.
Hull EM, Dominguez JM. Sexual behavior in male rodents. Horm Behav. 2007 Jun;52(1):45-55. doi: 10.1016/j.yhbeh.2007.03.030.
Stalnaya ID. Method for determining diene conjugation of unsaturated higher fatty acids. In: Orehovich VN, ed. Modern Methods in Biochemistry. Moscow: Medicine; 1977. p. 63-4. Russian.
Stalnaya ID, Garishvili TG. Method for determining of malonic dialdehyde using thiobarbituric acid. In: Orehovich VN, ed. Modern Methods in Biochemistry. Moscow: Medicine; 1977. p. 66-8. Russian.